ELISpot Plus: Mouse IL-6 (ALP)

ELISpot Plus: Mouse IL‑6 (ALP)

3361-4APW-2

Publications: 7
Documents
Questions?
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT26B2)
Detection mAb32C11, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse IL-6 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Mouse IL-6 (ALP)
ApplicationELISpot
AnalyteIL-6
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse IL-6.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells (50,000 cells/well) were incubated overnight with LPS or medium only and the number of cells secreting Interleukin-6 (IL-6) was analyzed by ELISpot.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IL-6

Analyte description

Interleukin 6 (IL-6) is a pleiotropic cytokine produced by many different cell types and plays a role in a wide range of functions, such as immune responses, acute-phase reactions, and hematopoiesis. Among other things, it augments antibody production from activated B cells in vitro.

Alternative namesInterleukin 6, IL-6, IL6, IFB-B502, BSF-2, BCDF, Il-6
Cell typeB cell, Monocyte/MΦ, mDC
Gene ID16193